A mounting medium with DAPI that is optimized for fluorescence microscopy with ibidi µ-Slides and µ-Dishes. Principle of the ibidi Mounting Medium With DAPI.
Principle of the ibidi Mounting Medium With DAPI
The aqueous, non-hardening ibidi Mounting Medium With DAPI is ideal for mounting immunofluorescence-stained samples that require counterstaining of the nucleus.

It is specifically designed to be used with ibidi µ-Slides and µ-Dishes. Being non-hardening, the ibidi Mounting Medium with DAPI also facilitates the mounting of samples in channel µ-Slides.

The ibidi Mounting Medium with DAPI contains a working concentration of DAPI (4',6-diamidino-2-phenylindole), which is a convenient DNA-binding fluorescent dye. This nucleus label is a robust nuclear/chromosomal counterstain, which is the gold-standard for immunofluorescence assays.

When bound to DNA, the fluorescence intensity of DAPI is much higher than when it is in solution. This enables direct fluorescence microscopy and the long-term storage of slides mounted with the ibidi Mounting Medium with DAPI.

With an excitation maximum of 358 nm and an emission maximum of 461 nm, the DAPI fluorescence signal exhibits a minimal spectral overlap with other frequently used fluorescence dyes in immunocytochemistry (e.g., GFP and similar dyes with even longer wavelengths).

• DAPI counterstaining and mounting combined in one single step
• Ready-to-use in immunofluorescence assays using widefield fluorescence and confocal microscopy
• Ready-to-use in immunofluorescence assays using widefield fluorescence and confocal microscopy
• Dropper bottle for exact dispensing into channels or open wells
• Brilliant fluorescence signals with low background fluorescence